WHEAT LEAF RUST GENOME SEQUENCING AND COMPARATIVE RESOURCES FOR RUST FUNGI
Cereal Disease Laboratory
2012 Annual Report
1a.Objectives (from AD-416):
The specific objectives of the proposal are:.
1)sequence and assemble the genome of the wheat leaf rust fungus, Puccinia triticina;.
2)annotate the genome using ESTs;.
3)publicly release all data on a comparative website (Broad) and NCBI; and.
4)provide training opportunities for students and postdocs.
1b.Approach (from AD-416):
Sequence and assemble the complete genome of P. triticina isolate 1-1, Race 1 (BBBD), which is estimated to be between 100 - 120 Mb, using a hybrid of 454 and ABI (Sanger) Fosmid-end sequence. Annotate gene structure using computational methods, 200,000 454 reads of ESTs from each of four new cDNA libraries, and other available ESTs. Evaluate P. triticina polymorphism and diversity by comparing the sequenced strain with three additional isolates using Illumina/Solexa sequence. Develop education, training and outreach programs.
This project is part of a larger program to compare the genomes of the three wheat rust fungal pathogens and develop genomic resources for rust fungi. This project is focused on the wheat leaf rust pathogen Puccinia triticina genome. Biological materials were developed from two sexual stages (pycnial and aecial) of wheat leaf rust pathogens that occur on the alternate host meadow rue (Thalictrum spp.). RNA was extracted and sequenced from the pycnial and aecial stages of the fungus. RNA sequence data was used to verify annotation of predicted gene calls in the P. triticina assembly. In addition, genes expression profiles were compared across different developmental stages of this fungal pathogen.