1a.Objectives (from AD-416)
The specific objectives of the proposal are:. 1)sequence and assemble the genome of the wheat leaf rust fungus, Puccinia triticina;. 2)annotate the genome using ESTs;. 3)publicly release all data on a comparative website (Broad) and NCBI; and. 4)provide training opportunities for students and postdocs.
1b.Approach (from AD-416)
Sequence and assemble the complete genome of P. triticina isolate 1-1, Race 1 (BBBD), which is estimated to be between 100 - 120 Mb, using a hybrid of 454 and ABI (Sanger) Fosmid-end sequence. Annotate gene structure using computational methods, 200,000 454 reads of ESTs from each of four new cDNA libraries, and other available ESTs. Evaluate P. triticina polymorphism and diversity by comparing the sequenced strain with three additional isolates using Illumina/Solexa sequence. Develop education, training and outreach programs.
This project is part of a larger program to develop genomic resources and analyze the genome of the wheat leaf rust pathogen. Studies of the genomes of rust pathogens have begun to provide insights into the mechanism by which these fungi cause disease. Biological materials were developed from the sexual stage of wheat leaf rust pathogen (Puccinia triticina) for verification of gene predictions in the annotated draft assembly. Characterization and comparison of the mitochondrial genomes of the wheat leaf rust pathogen and a closely related wheat stem rust fungus continues.
Regular e-mails and conference calls were used to monitor progress of this project.