1a.Objectives (from AD-416):
1. To analyze flavonoids in selected Scutellaria species.
2. To identify and isolate non-flavonoid anticancer compounds in selected Scutellaria species.
1b.Approach (from AD-416):
Scutellaria species will be grown at Fort Valley State University. Principal Investigator will harvest, dry, and powder tissue samples and will send powdered materials to collaborator at USDA, ARS, NPURU in Oxford, MS. Powdered samples will be extracted with methanol. The extract will be analyzed for flavonoid content using High Performance Liquid Chromatography (HPLC). The extracts will also be tested for activity to inhibit glioma cells. The extract with the strongest inhibitory activity will be further fractionated until an active compound is isolated. The isolated compounds will be structurally characterized using various spectroscopic methods (such as UV, IR, mass spectroscopy, nuclear magnetic resonance spectrometry).
Continuing work to isolate compounds from Scutellaria species, two compounds (one pink and one yellow) have been isolated from a sub-fraction that showed inhibition of brain cancer cells. The compounds are currently being tested to know whether or not they are the inhibitory compounds. Further isolation of these compounds is also being carried out, and chemical structure elucidated. A review paper covering biological and chemical aspects of this collaborative research work was published in Current Cancer Therapy.