1a.Objectives (from AD-416)
1. To analyze flavonoids in selected Scutellaria species.
2. To identify and isolate non-flavonoid anticancer compounds in selected Scutellaria species.
1b.Approach (from AD-416)
Scutellaria species will be grown at Fort Valley State University. Principal Investigator will harvest, dry, and powder tissue samples and will send powdered materials to collaborator at USDA, ARS, NPURU in Oxford, MS. Powdered samples will be extracted with methanol. The extract will be analyzed for flavonoid content using High Performance Liquid Chromatography (HPLC). The extracts will also be tested for activity to inhibit glioma cells. The extract with the strongest inhibitory activity will be further fractionated until an active compound is isolated. The isolated compounds will be structurally characterized using various spectroscopic methods (such as UV, IR, mass spectroscopy, nuclear magnetic resonance spectrometry).
Scutellaria ocmulgee leaf extract was fractionated following bioassay-guided fractionation. Sub-fractions have been tested for inhibition of proliferation of glioma cells. An active sub-fraction has been obtained. Isolation of the active compound is currently being performed. Work on this project was monitored by email. Also, ARS scientist visited with collaborators at their location at Fort Valley State University.