1a.Objectives (from AD-416)
Determine effects of parasitism by Ichthyophthirius or Gyrodactylus on fish susceptibility to Edwardsiella ictaluri and Flavobacterium columnare and develop diagnostic assays for detection of fish pathogen in cultured fish and farm water.
1b.Approach (from AD-416)
Catfish and tilapia will be used to evaluate the effect of parasitism by Ichthyophthirius multifiliis or Gyrodactylus on fish susceptibility to Edwardsiella ictaluri and Flavobacterium columnare; and determine if parasites can harbor and subsequently vector the bacterial pathogens using histology, fluorescent and molecular techniques. Rapid molecular and immunological detection assay will be developed and tested for their effectiveness in the detection of bacterial and parasite pathogens.
The goals of this NFCA are to develop rapid detection tests for fish pathogens, especially those pathogen that commonly occur concurrently. The early detection of fish pathogens will allow earlier intervention and thus limit the loss of fish produced in aquaculture. China is one of the major producers of seafood and the control of fish disease is very important to world security of aquaculture. No experiments have been initiated since this project started on March 1, 2010. Scientists of ARS and the institute have had discussion by phone to plan collaborative research in fish health between ARS and the institute. A site visit is sponsored by the cooperator for ARS scientists in 2010. Site visits and Emails will be used to monitor the progress of this NFCA.