1a.Objectives (from AD-416):
Test the ability of a fusion protein [lysostaphin fusion to the HIV TAT protein transduction domain] to cure chronic mastitis in cattle.
1b.Approach (from AD-416):
Field trial with chronically infected cows.
This work supports the National Program 101 mission statement in the area of developing information, tools, and technologies that can be used to improve animal production systems. Significant progress was made on Component 1: Understanding, improving, and effectively using animal genetic and genomic resources. Progress on this project focuses on Problem 1A, the need for developing genome-enabling tools and reagents for livestock (pig and cattle). These tools will not only be useful for traditional animal production research applications (reproduction, growth and development, nutrient intake and utilization, product quality), but will also be used to decrease the environmental footprint of animal production, improve animal health, well-being and resistance to disease, and enhance food safety. There has been significant advancement on this project to help cure chronic mastitis. Mastitis is a costly disease to the U.S. dairy farming with over $2 billion in losses to the industry annually. Progress has been made in developing a system for analyzing eradication of bacterial pathogens residing within cultured bovine mammary gland cells. Two unique enzyme antimicrobials harboring three unique cell wall degrading activities have been created and were fused to 11 different protein fragments that facilitate transport across the cell wall. These enzyme fusions were tested and optimal fusions identified for eradicating pathogens from within cultured mammary cells. This work will help to create antimicrobials to cure chronic multi-drug resistant pathogens and other drug resistant staphylococcal infections, such as osteomyelitis (bone infections) in humans.