Start Date: Feb 01, 2010
End Date: Dec 15, 2013
With respect to Objective 1, we will: 1. Serially passed BAC-cloned Marek’s disease virus (MDV) Md5 strain in triplicate and test for virulence. 2. Characterize genetic changes in the MDV genome using next generation sequencing, and correlate the allele frequency results with the level of virulence. 3. Characterize sequence changes that occur during the attention process using next generation sequencing, and correlate the expression differences with the level of virulence. 4. Determine SNP allele frequencies in MD vaccines. With respect to Objective 2, we will: 1. Validate changes identified in objective 1 but introducing defined mutations in our MDV-BAC clone.