OAT SNP DEVELOPMENT AND IDENTIFICATION OF LOCI AFFECTING KEY TRAITS IN NORTH AMERICAN OAT GERMPLASM____CROWN RUST TESTING
Small Grains and Potato Germplasm Research
2012 Annual Report
1a.Objectives (from AD-416):
Conduct a two year multi-location association mapping study to develop marker-trait associations to facilitate the development of new oat varieties with superior crown rust resistance.
1b.Approach (from AD-416):
Single row plots for all spring and CORE association lines will be grown in randomized complete block design with two replications. Disease severity data based on reactions to field isolates will be collected at the appropriate time. All data will be collected and analyzed for marker trait associations.
This is the final report for the project. During the life of the project, a total of 108 oat lines plus checks were evaluated for crown rust resistance in two replication and two years. Disease was inoculated three times in July enhance disease development. All the data were collected and sent to the CORE database for association mapping analysis. After analysis, this data may enable plant breeders to more efficiently screen for resistance to oat crown rust disease. Progress was made on the objective, which fall under National Program 301, Component 4: Plant Biological and Molecular Processes. According to the experimental design, the 108 CORE association lines plus 20 check lines were planted in Wisconsin location. Crown rust infection was evaluated for each line. The disease reading was conducted three times at July 12, July 18, and July 21. The final resistance score was recorded for each line and the data was sent to the CORE database. Results support objective 3 in the parent project plan, development of improved oat lines.