1a.Objectives (from AD-416)
To develop a panel of monoclonal antibodies that will be used to develop sensitive immunoassays for detection of multiple serotypes of botulinum neurotoxin.
1b.Approach (from AD-416)
Monoclonal anti-toxin antibodies for serotypes B and E will be developed using chemical cell fusion methods following immunization with toxoids, toxins, or recombinant peptides. In addition, antibodies to nontoxin associated proteins will be developed. The monoclonal antibodies will be supplied to DHS contractors and other collaborating federal laboratories in order to format sensitive immunoassays for toxin identification.
A cell repository for production of anti-ricin monoclonal antibodies has been established. Antibodies to BoNT serotype B and to some nontoxic associated proteins were developed. Production of antibodies to serotype E has begun. Assay development is underway.