1a.Objectives (from AD-416):
To develop a panel of monoclonal antibodies that will be used to develop sensitive immunoassays for detection of multiple serotypes of botulinum neurotoxin.
1b.Approach (from AD-416):
Monoclonal anti-toxin antibodies for serotypes B and E will be developed using chemical cell fusion methods following immunization with toxoids, toxins, or recombinant peptides. In addition, antibodies to nontoxin associated proteins will be developed. The monoclonal antibodies will be supplied to DHS contractors and other collaborating federal laboratories in order to format sensitive immunoassays for toxin identification.
In FY12 a cell repository for anti-ricin, anti-abrin, anti-botulism, and anti-SEA and SEB toxin has been established. An interlaboratory study was conducted to validate a newly developed immunoassay for ricin detection. Antibodies to BoNT serotype E were developed. Research progress report, addresses objectives 1 and 2 of the parent project.