1a.Objectives (from AD-416):
The objective of this research will be to evaluate the oat association mapping population for resistance to barley yellow dwarf virus (BYDV) and crown rust.
1b.Approach (from AD-416):
Hill plots for all 685 oat association mapping lines, along with spring and winter checks, will be grown in replicated field plots (three hills of 10 plants each spaced 30 cm apart; two hills infested with 30+ viruliferous (BYDV and CYDV) aphids and one hill noninfested (control). All hills, control and infested, will be covered with cups ventilated with fine mesh screen to prevent movement of aphids. Aphids will be destroyed 3-5 days after infestation. Plants within hills will be rated for tolerance to BYDV and data will be recorded. In addition 1-m-row plots, two replications, of each oat line will be grown for crown rust evaluations. The oat line P8669, known as susceptible to Puccinia coronata will be inoculated and will serve as disease spreader onto the test plots. Disease ratings will be collected at the appropriate times and data will be collected. All data will be uploaded and checked prior to association analyses.
This is the final report for the project. During the life of the project a total of 685 oat association mapping population lines were evaluated for barley yellow dwarf resistance and crown rusts resistance in Indiana. The experiment was repeated over two years with artificial inoculations. Data was collected for each of the lines and sent to the CORE database for association mapping. After analysis, this data may enable plant breeders to more efficiently screen for resistance to barley yellow dwarf and crown rust of oat. Progress was made on the objective, which fall under National Program 301, Component 2, Crop Informatics, Genomics, and Genetic Analyses. Progress on this project focuses on problem of assessment of disease resistance in oat lines and supports objective 3 in the parent project plan, development of improved oat lines. A total of 685 oat lines were evaluated for their resistance BYDV and CYDV. Data collected from the infected and non-infected treatments were uploaded to the database for association analysis.