1a.Objectives (from AD-416)
The objective of this research will be to evaluate the oat association mapping population for resistance to barley yellow dwarf virus (BYDV) and crown rust.
1b.Approach (from AD-416)
Hill plots for all 685 oat association mapping lines, along with spring and winter checks, will be grown in replicated field plots (three hills of 10 plants each spaced 30 cm apart; two hills infested with 30+ viruliferous (BYDV and CYDV) aphids and one hill noninfested (control). All hills, control and infested, will be covered with cups ventilated with fine mesh screen to prevent movement of aphids. Aphids will be destroyed 3-5 days after infestation. Plants within hills will be rated for tolerance to BYDV and data will be recorded. In addition 1-m-row plots, two replications, of each oat line will be grown for crown rust evaluations. The oat line P8669, known as susceptible to Puccinia coronate will be inoculated and will serve as disease spreader onto the test plots. Disease ratings will be collected at the appropriate times and data will be collected. All data will be uploaded and checked prior to association analyses. Documents Grant with Purdue University.
The 685 oat population developed under the USDA NIFA AFRI agreement 5366-21000-028-11R and North American Millers’ Association trust agreement 5366-21000-028-18T was planted in West Lafayette, IN. The population was infected with barley yellow dwarf virus using aphids. Disease ratings were collected for all 685 lines.
Communication and exchange of work has taken place via meetings.
The project is funded through a reimbursable agreement with USDA NIFA AFRI and North American Millers’ Association trust.