2011 Annual Report 1a.Objectives (from AD-416) The overall objective of this research is to develop protocols and methods for field-based high-throughput plant stress phenotyping. The specific ARS objectives are to genotype soybean accessions with molecular markers, produce high quality seed and assist in interpreting data. 1b.Approach (from AD-416) Isolate DNA from approximately 400 soybean accessions and then apply approximately 50 SSR and 50 SNP markers to each accession. Analyze marker data with emphasis on clustering. Increase seed of accessions as needed. Guide the project and interpret data. 3.Progress Report Currently high throughput genomics generates vast quantities of genetic and molecular information. However, the ability to similarly generate critical physiological data is limited. This research is designed to develop and evaluate high throughput physiological techniques and relate those to genomic information. Field experiments have been established in Arkansas and Missouri in cooperation with the University of Arkansas and the University of Missouri to evaluate a select group of almost 400 soybean genotypes. Molecular markers have been applied to over 300 genotypes.