1a.Objectives (from AD-416)
Linkage disequilibrium study on parents and C5 cycles of random mating using molecular markers. Marker data on parental lines have been completed. Development of recombinant inbred lines from half-sib families in the random mating.
1b.Approach (from AD-416)
Winter nursery will be utilized to increase seed of RIL to produce about one pound of selfed seed from each half-sib family. Seed will be planted for agronomic and fiber performance in a field environment.
We previously developed a random mated population beginning with a diallel of 11 parents composed of 11 very diverse lines involving 10 cultivars and one breeding line. These cultivars represented the major seed breeding companies and included cultivars from California to the Carolinas. This population was random mated for 5 cycles. The random mated seed were released to the public and registered with Crop Science Society of America as RMUP-C5. We have now developed 10 recombinant inbred lines (self pollinated by single seed decent for five generations) from each half sib family for a total of 550 recombinant inbred lines. These data are being used in association analysis to associate molecular markers with quantitative trait loci (QTL) for specific traits. Progress was monitored by frequent contacts by phone and e-mail as well as a meeting each year among all cooperators and Cotton Incorporated. Quarterly reports were provided to Cotton Incorporated.