SIRNA-MEDIATED KNOCKDOWN/SILENCE POST-TRANSCRIPTIONAL GENE EXPRESSION OF CHICKEN IFN-GAMMA
Animal Biosciences and Biotechnology Laboratory
2013 Annual Report
1a.Objectives (from AD-416):
To explore the possibility of initial stage of host gene manipulation by developing RNA interference technology using primary lymphocytes. We will use RNAi technology to modulate IFN-y response since IFN-y is a key immunoregulatory cytokine of macrophages for mucosal immune response.
1b.Approach (from AD-416):
Peripheral blood monocytes will be cultured in vitro and treated with siRNA using recently developed RNA interference (RNAi) technology to knockdown/silence post-transcriptional gene expression of chicken IFN-y.
RNA interference technology was developed to decrease the expression of the chicken gene which encodes IFN-gamma in an established chicken macrophage cell line. However, this cell line did not show stability and lost its ability to continuously produce chicken IFN-gamma. The collaborating scientists are now exploring an alternative strategy to knockdown/silence the gene expression of chicken IFN-gamma.