Mode of Action of Antidiabetic Activity, Identification of Bioactive Compound and Micropropagation of Serviceberry (Amelanchier Alnifolia)
Natural Products Utilization Research
2013 Annual Report
1a.Objectives (from AD-416):
1. Identify mechanisms that mediate anti-diabetic effects of serviceberry extracts.
2. Isolate and identify the anti-diabetic constituent(s) from serviceberry.
3. Develop a robust micropropagation system for rapid germplasm multiplication, maintenance and storage.
4. Develop efficient organic production systems for serviceberry cultivation.
1b.Approach (from AD-416):
ARS contact scientist will identify anti-diabetic constituents from serviceberry through activity-guided fractionation using an assay that will be conducted at Auburn University, another collaborator in this project. ARS scientist will use various spectroscopic methods to identify the anti-diabetic constituent(s).
Progress has been made in the isolation of a compound from the water soluble fraction of Serviceberry leaf extracts, which showed activity against alpha-glucosidase, a target enzyme for anti-diabetic medications. However, this compound, which appears to be polymeric, is unstable and rendered itself insoluble hindering further chemical structure characterization. It was found not suitable to pursue further chemical work on this compound due to its chemical instability. The ethyl acetate soluble fraction of Serviceberry leaf extracts was then investigated, and further fractionation has shown one fraction that has good inhibition of alpha-glucosidase. Sub-fractionation is being done, and subfractions will be sent to Auburn University collaborator for assay.