1a.Objectives (from AD-416)
1. Develop and validate diagnostic tests for the viruses involved in these complexes and transfer these validated tests to interested parties; 2. Identify candidate virus vectors based on virus genomics with greenhouse transmission testing; 3. Identify virus combinations capable of causing severe disease outbreaks, and; 4. Evaluate virus and vector resistance in Rubus germplasm; conduct field transmission tests to determine when viruses are being spread in the field and implement targeted control based on vector biology for management of the diseases. 5. Communicate the results: Outreach, Education and Implementation for growers, extension agents, and agricultural consultants.
1b.Approach (from AD-416)
This project aims to minimize or eliminate the impact of virus complexes in production fields through minimal pest management targeting the virus/vector combinations easiest to control, this includes the improvement of virus testing in certification programs. Documents Reimbursable with U of AR (CSREES-Specialty Crops Research Initiative). Log 39156.
Research field plots to study the impact of raspberry bushy dwarf, raspberry leaf mottle and raspberry latent viruses have been established in northern Washington on the WSU-Mt. Vernon research station and in Corvallis, OR at the ARS-NCGR farm on Peoria road. Plants infected singly with each of the viruses as well as all virus combinations have been established in replicated plots (5 replications per site). In addition, on farm trials have been set up to examine the time of aphid emergence and major flights in the field. These plots include examining parasitism and predation of the aphids. Sticky traps for aphids, thrips and leafhoppers have also been placed in each field. Farm field studies are also being conducted to examine the rate of infection with each of these three viruses in newly established plantings of raspberries. These plots include aphid susceptible and aphid resistant cultivars. Farm plots have also been established to study the rate of nematode transmission of Tomato ringspot virus after various types of site preparation for nematode control prior to planting. Molecular characterization of a new reovirus in raspberry is nearly completed and transmission studies are underway in greenhouse/growth chamber studies.