1a.Objectives (from AD-416)
Better understanding of the environmental and host factors that influence antibiotic resistance gene transfer and utilize this information to formulate more sustainable ways of using antibiotics in agriculture.
1b.Approach (from AD-416)
A green fluorescent protein-tagged derivative of an IncA/C plasmid, developed by the ARS PI, will be utilized to assess the in vivo host range of this plasmid family. This tool will also used to quantitatively assess the effect of antibacterial treatment on the maintenance and spread of this family of plasmids within the fish gut and within fish culture systems. Bacteria which have acquired this plasmid will be identified by virtue of the GFP tag and identified to species by 16S rDNA analysis.
This project involves collaborative research planned for 2011-2012. Preliminary experiments performed this year have verified that the green fluorescent protein-tagged IncA/C plasmid derivative constructed for this project has retained its capability for self-mobilization and has retained expression of its antimicrobial resistance profile. Methods for the visual detection of bacterial strains containing the plasmid have also been developed. The ADODR has also stayed in contact with the cooperator for planning of the upcoming effort. This has involved discussions of the research approach and review of the visiting student’s progress in 2011 as well as planning for the students travel and stay while in the US.
Progress is monitored through email and conference calls.