1a.Objectives (from AD-416)
Better understanding of the environmental and host factors that influence antibiotic resistance gene transfer and utilize this information to formulate more sustainable ways of using antibiotics in agriculture.
1b.Approach (from AD-416)
A green fluorescent protein-tagged derivative of an IncA/C plasmid, developed by the ARS PI, will be utilized to assess the in vivo host range of this plasmid family. This tool will also used to quantitatively assess the effect of antibacterial treatment on the maintenance and spread of this family of plasmids within the fish gut and within fish culture systems. Bacteria which have acquired this plasmid will be identified by virtue of the GFP tag and identified to species by 16S rDNA analysis.
This project involves collaborative research planned for 2010-2011 in which a Norwegian graduate student will be trained and work in the laboratory of the ARS PI. A graduate student was selected for this work and a Research Plan has been written and approved by his thesis committee. The ADODR is in frequent contact with the cooperator through phone calls, email, and annual site visits in addition to receipt of written reports.