2010 Annual Report
1a.Objectives (from AD-416)
This project will integrate research, extension, and education activities to develop new breeding material or improved breeding tools in common bean, with the goal of enhancing nutritional traits in this crop. An association mapping population consisting of 400 diverse lines, representing the major US market classes, will be evaluated for a number of nutritional traits including minerals, protein, carbohydrates, fiber, vitamins, health-beneficial phytochemicals, and differences in iron bioavailability. All of the lines will be genotyped, using currently available molecular markers, and the phenotype and genotype data will be used to discover regions of the genome that affect the nutrient traits. This will be accomplished using modern association mapping techniques, and will lead to the development of readily scorable molecular markers that bean researchers can use to breed new bean cultivars with enhanced nutritional quality.
1b.Approach (from AD-416)
Mineral concentration and iron bioavailability studies will occur on seed samples of common bean using ICP-OES (inductively coupled plasma – optical emission spectroscopy) or an in vitro model system (Caco-2 cells). The CNRC will analyze approximately 9,600 bean samples using ICP-OES and as many as 600 samples using our Caco-2 iron bioavailability model.
Our major activities in this project will be to analyze mineral concentrations and iron bioavailability potential in the dry bean and snap bean samples that will be shipped to us by other cooperators. As of this reporting period, no samples are yet available. However, in anticipation of the first samples due to arrive in late 2010, we have been assessing various software and hardware configurations that will allow us to document and inventory the samples. In the past, we have used simple numerical and lettering identifiers for all samples, and spreadsheets to record information on sample identity and storage location. However, all of our past experiences have usually involved unique samples. For this project, in which the same genotypes will be grown in different locations and different years, we wish to ensure sample identity by location by year throughout the project, especially to allow us to return to samples for additional analyses if these are required. Thus, we are evaluating off-the-shelf software packages and bar code readers to purchase and use with this research project.
Regarding our analytical techniques, we have established new program parameters on our ICP-OES (inductively coupled plasma – optical emission spectrometer) that will allow us to include selenium (Se) in our mineral analyses. New standard solutions have been purchased that include Se, along with our other minerals to be assayed; these will be used for instrument calibration. For the Caco-2 in vitro cell culture studies to assess iron bioavailability, we have been discussing how to handle the small scale processing of samples (e.g., cooking methods) prior to the analyses, and how best to pilot test various procedures.
The cooperative parties discuss the activities and progress of the project through e-mails and phone calls, as well as via in-person meetings with the Project Executive Committee and Project Advisory Committee. Progress reports for all facets of the project were presented to the Advisory Committee; this Committee subsequently gave various management recommendations to the PD and the Executive Committee.