2010 Annual Report 1a.Objectives (from AD-416) Develop and apply marker assisted breeding in Rosaceae fruit crops more efficiently and rapidly. 1b.Approach (from AD-416) We have organized a diverse team of researchers (genomicists, breeders, ag economists, and extension personnel)to: 1)increase the likelihood of new Rosaceae cultivar adoption, 2)integrate breeding and genomics resources by establishing a standardized statistical framework and breeding information management system, 3)establish sustainable infrastructure for an efficient MAB pipeline in Rosaceae including crop-specific SNP genome scan platforms for breeding-relevant germplasm, 4)implement the MAB pipeline to demonstrate application of efficient MAB schemes in U.S. breeding programs, and 5)enhance sustainability of cultivar development by training of current and future Rosaceae plant breeders and active engagement of industry stakeholders and the allied research community. Specialty Crop Research Initiative. Documents reimbursable with Michigan State U. Log 38887. 3.Progress Report DNA sequences from genotypes of peach (23), sweet cherry (16), sour cherry (8), apple (10) and strawberry (3) were generated. Bioinformatics analysis identified single nucleotide polymorphisms in peach and cherry. Tissue samples from peach, cherry, and apple were obtained and processed for DNA extraction. Progress is being monitored through monthly conference calls, e-mail, website updates (www.rosbreed.org) and at scientific meetings.