UTILIZING GENETICS AND PHYSIOLOGY FOR ENHANCING COOL AND COLD WATER AQUACULTURE PRODUCTION
Cool and Cold Water Aquaculture Research
Project Number: 8082-31000-010-00
Start Date: Jan 11, 2010
End Date: Jan 10, 2015
1: Define phenotypic measures and estimate genetic and phenotypic parameters and correlations for production, product quality, and reproductive quality traits.
• 1.a. Estimate genetic and phenotypic parameters and correlations for
production and product quality traits.
• 1.b. Estimate genetic parameters and correlations for reproduction traits.
• 1.c. Evaluate accuracy of live-animal ultrasound measures to predict body
composition and fillet quality.
2: Identify physiological basis for variation in, and strategies to improve growth and nutrient utilization in rainbow trout.
• 2.a. Identify regulatory mechanisms for nutrient utilization in muscle and liver.
• 2.b. Identify genetic variation in expression of regulatory proteins within nutrient signaling pathways.
• 2.c. Identify and select on genetic markers for growth (feed) efficiency.
3: Identify physiological basis for variation in, and strategies to improve reproductive performance in rainbow trout.
• 3.a. Identify growth factors that affect final maturation and their signaling pathways.
• 3.b. Identify effects of the maturation-inducing hormone MIH, candidate growth factors and signaling pathways on translation of maternal proteins during follicle maturation and in response to fertilization.
• 3.c. Identify changes in germ cell transcription of TGF-beta superfamily growth factors during oogenesis and oocyte recruitment with the aid of transgenic trout carrying a green fluorescent protein gene driven by the vasa gene promoter (GFP-vasa).
4: Improve procedures for natural triploid (2Nx4N) production and evaluate their performance.
• 4.a. Evaluate performance of natural triploid (2Nx4N).
• 4.b. Improve procedures for natural triploid (2Nx4N) production.
A comprehensive multidisciplinary strategy utilizing quantitative genetic, physiological and molecular biological approaches is being used to produce genetically superior strains of rainbow trout for release to trout producers, and to develop the technologies for rapid and continued innovation and improvement. As part of this research we will continue to evaluate and characterize the broodstock established at the NCCCWA selected for improved growth performance. Offspring from this line of rainbow trout will be evaluated for important aquaculture production traits e.g., growth, feed efficiency, and reproductive development. These data will yield estimates of additive genetic variation among and within families of rainbow trout and provide guidance for designing selection and breeding programs for genetic improvement, including strategies that employ the use of molecular markers. Physiological research will focus on defining critical pathways, and molecular components in those pathways, for economically important traits. Furthermore, animals with extreme phenotypes, identified by quantitative genetic analyses, will be used in physiological studies to define the critical physiological differences. Procedures for tetraploid induction will be improved for the development of natural triploids and this technology will be applied to standard and improved lines to evaluate its potential to provide additional benefits to rainbow trout aquaculture.