1a.Objectives (from AD-416)
1) Identify new sources of resistance and introgress resistance genes into desirable agronomic backgrounds;. 2)Phenotype mapping populations for developing new DNA markers and confirm resistance.
1b.Approach (from AD-416)
Screen soybean germplasm using standardized methods in greenhouse assay. Phenotype mapping populations from other scientists. Evaluate for presence of DNA markers using PCR reactions for Marker assisted selection.
Application of Biotechnology to control soybean cyst nematode (SCN): Disease Resistance Genes is a collaborative research project funded by the United Soybean Board (USB) via SIU. For broadening the resistance in soybean, additional soybean germplasm is being screened for identifying new sources of resistance to SCN. Genetic mapping populations are also being screened (phenotyped) in the greenhouse to confirming resistance and to develop new DNA markers associated with resistance derived from PI438489B. Soybean PI438489B is a new introduction from P.R. China whose resistance to multiple nematode populations was discovered at ARS Jackson, TN. Since it is not desirable commercially, PI438489B was crossed to the cultivar Hamilton to develop progenies. These progenies are used for developing DNA markers and release of desirable source material for resistance. ARS laboratory in Jackson, TN worksite is the center for screening soybean for nematode resistance under the USB funded collaborative research project. The greenhouse screening and developing desirable source materials are in progress, and the data are shared. ADODR monitored the research primarily through meetings, site visits, emails and conference calls.