Rift Valley Fever Diagnosis and Vaccine Development and Evaluation
Arthropod-Borne Animal Diseases Research
2011 Annual Report
1a.Objectives (from AD-416)
The objective will be to develop, expedite the evaluation and standardization of Rift Valley fever (RVF) diagnostic assays and vaccine control strategies.
1b.Approach (from AD-416)
This project will utilize the vector-borne disease expertise of the ARS, Arthropod-Borne Animal Diseases Research Unit (ABADRU) and the Rift Valley fever (RVF) diagnostic expertise of the Agricultural Research Council (ARC) to develop, evaluate, and standardize diagnostic assays and vaccines for RVF. The approach will be to develop a young ruminant animal challenge model to evaluate existing and newly developed vaccines and diagnostics for RVF. The collaborative project will include the evaluation of existing and newly developed diagnostic tests such as various viral genome amplification assays (real-time RT-PCR, RT-LAMP, and LATE-PCR) and enzyme-linked immunosorbent assays (ELISA) based on recombinant antigens (RVF-N, -G2, and –NSs). This will be achieved by direct comparison of available assays in both field-deployable and diagnostic laboratory based formats. The goal is to provide recommendations for standardized diagnostics that can be universally applied by international disease outbreak response teams. The collaborative project will also evaluation of the host immune response to new vaccines and virus challenge.
This project was initiated to develop, expedite the evaluation and standardization of Rift Valley fever (RVF) diagnostic assays and vaccine control strategies. This project resulted in generating important supportive data for the ARS RVF triplex assay that would not have been easy to obtain otherwise. The prototype lateral flow serological and antigen capture assays all performed very well.
This research supports NP103 Action Plan Components 1. Biodefense Research, and 3. Prevent and Control Zoonotic Diseases.
ADODR is directly involved in performance of the research and also monitors activities to evaluate research progress through site visits, meeting at conferences and through email and phone calls.