2012 Annual Report
1. Perform ex situ VOC analysis on a large portion of selected oak tree (Quercus spp.) leaves to determine general VOC emission trends.
2. Utilize the in situ small-scale collection method (collection bag, SPME) of the USDA-ARS PMR unit to acquire VOCs from select trees identified from ex situ analysis.
3. Separate and identify collected VOCs via GC-MS analyses.
4. Develop a chemotaxonomic tree based upon trends of identified VOCs and herbivore damage.
5. Utilize electrophysiological and/or behavioral bioassays to determine kairomonal activity. Documents NFCA with UC Davis.
The project was extended past the first year to allow the graduate student (now graduated with a Ph.D.) to write up the results. A manuscript entitled “Headspace volatiles from 52 oak species advertise induction, species identity, and evolution, but not defense” was recently submitted (7/17/2012) to the Journal of Chemical Ecology. The UCD scientist involved with this project is working on a second paper detailing the results of this project.
The goals of this project, collecting and identifying volatiles as potential kairmones for oak insect pests, are related to objective 3 of the in-house project and are a direct application of methods developed in this laboratory.