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United States Department of Agriculture

Agricultural Research Service

Research Project: VOLATILE EMISSION ANALYSIS OF SEVERAL CALIF OAK SPECIES: DO EMITTED VOLATILES EXHIBIT KAIROMONAL BEHAVIOR TO VARIOUS OAK INSECT PESTS?
2011 Annual Report


1a.Objectives (from AD-416)
1. To collect the ex situ and/or in situ volatile emission of select native and non-native oak trees (Quercus spp.). 2. To analyze collected volatile organic compounds (VOCs) via GC-MS and identify possible chemotaxonomy trends and/or chemotype identifiers of selected trees to various insect pests. 3. To identify VOCs with kairomonal behavior to select insect pests.


1b.Approach (from AD-416)
To accomplish the stated objectives the following approach will be utilized by the graduate student under the mentorship of a Professor and ARS scientist.

1. Perform ex situ VOC analysis on a large portion of selected oak tree (Quercus spp.) leaves to determine general VOC emission trends.

2. Utilize the in situ small-scale collection method (collection bag, SPME) of the USDA-ARS PMR unit to acquire VOCs from select trees identified from ex situ analysis.

3. Separate and identify collected VOCs via GC-MS analyses.

4. Develop a chemotaxonomic tree based upon trends of identified VOCs and herbivore damage.

5. Utilize electrophysiological and/or behavioral bioassays to determine kairomonal activity. Documents NFCA with UC Davis.


3.Progress Report

All volatile collections have been performed, the volatiles separated and identified via gas chromatography mass spectrometry GC-MS, and the data are currently being analyzed.


Last Modified: 10/23/2014
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