2010 Annual Report 1a.Objectives (from AD-416) Develop SNP markers for soybean aphid resistance genes for marker assisted breeding for the Rag2 gene. 1b.Approach (from AD-416) SNP (single nucleotide polymorphism) markers will be identified in the genomic regions of the aphid resistance genes using appropriate RIL mapping populations and near isogenic lines. The Solexa sequencing and Illumina and other genotyping platforms will be used for mapping the SNPs. 3.Progress Report Rag2 is being fine mapped using 400 RIL (F5:6) lines of the Wyandot x PI 243540. We are mapping 7 SSR and 10 SNP marker in a 3 cM region flanking the Rag2 gene on linkage group F, these SNPs were identified via Solexa sequencing of Rag2 NILS using reduced representation library. Phenotyping of the RILs was completed in May, and genotyping of the Rag2 region is currently underway. This research is being done on the Wooster campus of The OSU at the same location of ADODR. Research progress is reviewed on a monthly basis with the Co-PI and technical personnel working on the project. Also, a quarterly written report on progress is submitted by the Co-PI to the ADODR.