1a.Objectives (from AD-416):
1. Determine metabolic pathways that have a direct or indirect role in CO2 injury inception and development.
2. Identify metabolites that may be useful for predicting or diagnosing CO2 injury during the pre-symptomatic stages.
3. Test the capacity of prospective metabolic biomarkers to predict or diagnose CO2 injury under different storage conditions known to affect injury occurrence or severity.
1b.Approach (from AD-416):
Apple fruit will be treated and stored under conditioned known to provoke or reduce scald. Broad untargeted metabolic profiling techniques will be used to track symptom develop from disorder inception, through the asymptomatic development stages, and finally, during symptom development in fruit that are expected to, or not expected to, develop symptoms. Metabolomic data will be compiled and modeled using multivariate data-mining techniques to reveal associations among metabolites from multiple pathways and symptom development. Further experimentation will employ further treatment and storage-based contrasts that are expected to link other metabolites to disorder development while validating already discovered prospective metabolic biomarkers.
This serves as a final report for project 5350-43000-006-10T which expires 10/31/2013. This project relates to objective 2 of the associated in-house project which seeks to identify factors that influence postharvest fruit quality and development of market limiting physiological disorders. Chemical analysis of ‘Empire’ apple peel stored in conditions that typically produce crop loss from peel damage identified natural compounds in the fruit that may be indicators of injury risk prior to symptom development. Some of these fruit compounds may also distinguish CO2 injury from other disorders with similar appearance that are caused by different conditions during storage. Metabolites associated with this disorder include several associated with fermentation all of which can be measured in the storage headspace to monitor disorder development. With additional validation, this knowledge may be used to develop commercial processes to assess risk of CO2 injury while apples are in storage and to identify fruit that have developed CO2 injury.