Location: Plant Gene Expression Center Albany_CA
2011 Annual Report
The effects of hormones on miR172 expression were examined using microRNA northern blots. miR172 levels were greatly decreased in the shoot apices of several GA biosynthetic maize mutants as well as mutants defective in GA response. These results indicate that GA biosynthesis and perception act upstream of miR172 to activate its expression. The promoters of several miR172 genes in grasses and dicots are currently under analysis to identify common GA response elements active during the juvenile phase of development.
Chromatin immunoprecipitation was used to identify targets of the AP2 gene branched silkless1 (bd1) based on the presence of bd1 binding sequences in their promoters. Preliminary evidence suggests that another AP2 gene, indeterminate spikelet1, may be a target of bd1. In addition, a second transcription factor responsible for floral initiation called zmLFY also appears to be a target. High through put sequencing of ChiP DNA is currently being used to identify additional targets of bd1.