Location: Cool and Cold Water Aquaculture Research
Project Number: 8082-32000-005-01
Start Date: Jun 04, 2010
End Date: Jan 01, 2014
Improved germplasm from ongoing ARS NCCCWA programs and breeding populations under investigation will be established in small and large scale field trials. In the small-scale trial, 25,000 eyed eggs will be produced from two NCCCWA fish lines: ARS-Fp-R (resistant) and ARS-Fp-S (reference) fish. Eyed eggs will be shipped to a Clear Springs Foods, Inc. and subsequently hatched and reared under standard production conditions. This experiment will evaluate three objectives: 1) production and shipping of eggs; 2) obtain baseline/comparative production and survival data; and 3) investigate the incidence of BCWD infection. A large-scale field trial will include 70,000 fish from the ARS-Fp-R line (resistant); 35,000 fish from the ARS-Fp-C line (control line); and 35,000 fish from the ARS-Fp-S line (reference). All fish will be reared in a single production lot in order to ensure a uniform environment and equal pathogen exposure. Farm performance evaluation will consist of recording daily raceway mortality, monitoring clinical disease symptoms and kinetics including lesions and splenomegly, determining pathogen load in internal organs (by microbiological plating and quantitative PCR), and measurement of fish weight and length. Fish group will be determined by fin-clip with parental assignment made by PCR-based genotyping.