2012 Annual Report 1a.Objectives (from AD-416): To determine the effect of increasing animal source food intake on the nutritional status of Kenyan women and their children. 1b.Approach (from AD-416): Serum samples will be collected by Kenyan staff hired by Indiana University in Kenya. The samples will be analyzed for micronutrient content at the WHNRC, using radioassays and high pressure liquid chromatography. 3.Progress Report: This research contributes to Objective 2A of the in-house parent project. HIV positive Kenyan women and their infants (n=290 mother-infant pairs) have been enrolled in an international study and the mothers supplemented with meat, soy or wheat protein for a year to evaluate whether supplements lower viral regression and improve micronutrient status. The feeding trial ended in April of 2011 when blood samples had been taken at 5 time points from the women and their children. Samples were shipped to the WHNRC in August 2011. In FY12 4800 samples were inventoried for multiple nutrient analyses. Analyses were completed for vitamin B12, folate, and zinc. Vitamin A, C-reactive protein, alpha-1 acid glycoprotein and transferrin receptor analyses are in progress. The final set of samples is due to arrive at the end of August 2012, and all analyses will be completed by the end December 2012. Data analysis and publication will start in 2013.