2010 Annual Report 1a.Objectives (from AD-416) Application of new chemical therapies to eliminate Huanglongbing (HLB) bacterium from HLB-infected citrus plants. 1b.Approach (from AD-416) Implementation of chemical control strategies by applying new commerical device to deliver effective chemicals into citrus plants. Evaluation of the control efficacy of the treated citrus plants using PCR-based detection. 3.Progress Report This research relates to inhouse objective 3: Develop or improve comprehensive integrated disease management strategies. The survey of Murraya plantings in St Lucie and Palm Beach counties is ongoing. In on our early sampling, Ca. Liberibacter asiaticus is present in Murraya at all seven urban plantings. Ca. L. asiaticus was detected in 3.4% - 23.3% of the samples taken at each site, although the titer of the bacteria was uniformly low. The psyllid population was low in our spring sampling, but we detected Ca. L. asiaticus in 5.3% of all the psyllids we collected. The bacteria was detected in psyllids from three sites, with the average prevalence ranging from 0 - 10.5% according to site. Like the Murraya plants, the bacterial titer of the infected psyllids was low. Monitoring was accomplished by phone calls, emails and site visits.