2011 Annual Report
The research included collaborative development of assay technologies for detection of botulinum neurotoxins (BoNTs) in food and sera. Monoclonal antibodies against BoNT/A and BoNT/B were optimized for the detection of toxin in sera using a multiwell plate-based electrochemiluminescence (ECL) assay system. Pharmacokinetics of BoNTs were studied in sera using the ECL assay system. Collaborators provided humanized therapeutic antibodies for testing of toxin neutralization and expertise in new assay technology for the detection and visualization of toxin in organs. Collaboration is facilitated by annual meetings of the Pacific Southwest Regional Center of Excellence, e-mail, and ad hoc meetings with collaborating laboratory personnel. Manuscripts have been prepared and yearly renewal reports are submitted to the granting agency (DHHS-PHS-NIH National Institute of Allergy and Infectious Diseases).