Project Number: 5442-21000-033-17
Project Type: Reimbursable

Start Date: Oct 01, 2009
End Date: Aug 31, 2012

Objective:
The main objectives of the proposal are to develop single nucleotide polymorphism (SNP) markers from gene regions of the wheat genomes, to construct SNP marker-based genetic maps and to study genetic diversity among U.S. wheat cultivars using a high throughput genotyping assay system.

Approach:
1. The source of SNPs will be from cDNAs derived from the gene regions of the wheat genomes. Random cDNAs produced from a panel of 16 diverse wheat cultivars will be subjected to a large-scale sequencing using the next-generation sequencing instrument GX FLX developed by Roche. 2. SNPs will be discovered from the analysis of large-scale sequence data through the use of bioinformatics tools. 3. The SNP information will then be used to develop high-throughput genotyping assays based on the methods developed by Illumina. 4. The genotyping assays will be performed to construct SNP marker-based genetic maps and to evaluate genetic diversity among U.S. wheat cultivars.