1a.Objectives (from AD-416)
We will conduct fine mapping of the Rag2, Rag3, Rag4 genes for soybean aphid resistance with SNP markers. Also identify candidate genes and proteins for the Rag2 gene identified earlier by ARS.
1b.Approach (from AD-416)
To develop SNP markers for soybean aphid resistance genes for marker assisted breeding for Rag2, Rag3, and Rag4 genes: SNP markers will be identified in the genomic regions of the aphid resistance genes using appropriate RIL mapping populations (already in hand) and near isogenic lines. The Solexa sequencing and Illumina genotyping platforms will be used for this objective/strategy.
We developed the backcross and near isogenic lines needed for fine mapping of the Rag2 gene. Then infested the isogenic lines with the soybean aphid biotype 2 and collected the tissue from the infested plants to be used for fine mapping of the Rag2 gene. Similarly, infested the near isogenic lines with soybean aphids and collected plant tissue at different time points for transcriptomics and proteomics studies. Also, coordinated the fine mapping of Rag 3 and Rag 4 and transcriptomic and proteomic research.
This research relates to objective 2A (Characterize and map aphid resistance genes in three soybean plant introductions) of the parent project.