1a.Objectives (from AD-416)
We will conduct fine mapping of the Rag2, Rag3, Rag4 genes for soybean aphid resistance with SNP markers. Also identify candidate genes and proteins for the Rag2 gene identified earlier by ARS.
1b.Approach (from AD-416)
To develop SNP markers for soybean aphid resistance genes for marker assisted breeding for Rag2, Rag3, and Rag4 genes: SNP markers will be identified in the genomic regions of the aphid resistance genes using appropriate RIL mapping populations (already in hand) and near isogenic lines. The Solexa sequencing and Illumina genotyping platforms will be used for this objective/strategy.
Mapping of Rag2 gene is in progress under a SCA with Ohio State University. We grew RIL mapping populations, maintained soybean aphid biotypes, collected plant tissue and provided those to the Co-PI for mapping. Mapping of Rag3 and rag4 genes are in progress at Michigan State University under an SCA (5S). Proteomics study is being conducted at University of Missouri Columbia under an SCA (6S). We maintained the soybean aphid, treated plants with soybean aphids, collected plant tissue and provided those to the Co-PI.