2010 Annual Report 1a.Objectives (from AD-416) To control the soybean cyst nematode using new approaches including molecular biology and transformation techniques. 1b.Approach (from AD-416) DNA vectors will be designed for transformation of DNA fragments into soybean to control the soybean cyst nematode (SCN) so that it will not grow or will not complete its life cycle. The transformed soybean roots will be tested to determine the effect of the DNA fragments on SCN vigor. 3.Progress Report The objective of this agreement is the development of molecular technologies leading to soybean with greater resistance to the soybean cyst nematode. At least ten candidate genes were cloned and sequenced. Promoters of two genes were cloned and a 1 kb region was sequenced from each promoter. This is funded by the United Soybean to work on developing soybean with resistance to the soybean cyst nematode. Quarterly progress reports are filed with the United Soybean Board. An annual meeting is organized by the United Soybean Board and held every February. Conference calls and e-mails are also used to monitor and coordinate progress.