1a.Objectives (from AD-416)
To develop sorghum lines with high lignin content in order to test the hypothesis that higher lignin will increase bioenergy available through combustion.
1b.Approach (from AD-416)
Transgenes expected to increase lignin content will be inserted into the sorghum genome. Transformed lines will be evaluated for lignin content and for energy released during combustion.
Seventeen successful transformation events have been accomplished. First generation plants are currently growing in the greenhouse. Two additional constructs have been assembled and delivered to the cooperator who is currently attempting to insert those constructs into sorghum. All constructs contain genes expected to increase lignin and total energy content of the resulting transformed lines. Efforts to transform the forage variety ‘Atlas’ proved unsuccessful. All transformation events were made in the proven parental line RTx430. Monitoring of the project was accomplished by regular on-site visits and telephone conversations with the cooperator.