1a.Objectives (from AD-416):
To develop sorghum lines with high lignin content in order to test the hypothesis that higher lignin will increase bioenergy available through combustion.
1b.Approach (from AD-416):
Transgenes expected to increase lignin content will be inserted into the sorghum genome. Transformed lines will be evaluated for lignin content and for energy released during combustion.
Five transgenes were designed and constructed to increase the amount of the cell wall polymer, lignin, in sorghum biomass intended for use in combustion or for conversion to liquid fuels via pyrolysis. These transgenes have been inserted into sorghum and the transformed lines are being evaluated for their ability to increase lignin concentration in sorghum biomass. The five transgenic sorghum lines represent the experimental foundation for exploring the potential to engineer increased lignin content through biotechnology in sorghum and unlock this potential resource for the U.S. sorghum and bioenergy industries.