2011 Annual Report 1a.Objectives (from AD-416) Deployment of nematode resistant carrots to U.S. growers and seed industry. 1b.Approach (from AD-416) We have discovered multiple genetic sources of nematode resistance in carrot germplasm. In this project we will confirm the strength of the resistance, intercross best resistance, produce ample seed for extensive field testing, and release best resistance to the public, especially growers and seed companies. We will also evaluate horticultural quality including field performance traits (color, shape, uniformity) and consumer quality (flavor and nutritional value), hold field days for the carrot production community, and confirm genetic models of resistance in mapping populations. 3.Progress Report United States (U.S.) carrots are an important source of nutrients and farm income, but yield can be seriously reduced by attack of root-knot nematodes. New sources of genetic resistance to the two most important root-knot species affecting carrot production are under development. This project will provide field testing of new carrots with nematode resistance in a major U.S. production area, Kern County, California. Trials were evaluated for horticultural quality (shape, color, smoothness, uniformity, and flavor) and stock roots for laboratory evaluation of nutrients – carotenoids and anthocyanins were delivered to Wisconsin for lab evaluation. Project monitored by regular meetings and e-mail discussions of progress.