2012 Annual Report 1a.Objectives (from AD-416): Deployment of nematode resistant carrots to U.S. growers and seed industry. 1b.Approach (from AD-416): We have discovered multiple genetic sources of nematode resistance in carrot germplasm. In this project we will confirm the strength of the resistance, intercross best resistance, produce ample seed for extensive field testing, and release best resistance to the public, especially growers and seed companies. We will also evaluate horticultural quality including field performance traits (color, shape, uniformity) and consumer quality (flavor and nutritional value), hold field days for the carrot production community, and confirm genetic models of resistance in mapping populations. 3.Progress Report: Nematode resistance was evaluated in the field and greenhouse where performance was consistent with earlier evaluations. Evaluation of genetic mapping populations provided the basis for identifying a new gene for resistance under additional analysis. This research relates to Objective 1, Determine the genetic basis of and initiate selection for carrot, onion, cucumber, and melon quality attributes influencing human nutrition and health, disease resistances, and yield and quality components, and stress tolerance in cucurbits, and perform field performance and quality trials; Objective 2, Utilize current biotechnology to discover and evaluate genetic variation and to map agriculturally important traits in Allium, Cucurbit, and Daucus germplasm, and to develop genetic and breeding stocks.