2012 Annual Report
1a.Objectives (from AD-416):
Deployment of nematode resistant carrots to U.S. growers and seed industry.
1b.Approach (from AD-416):
We have discovered multiple genetic sources of nematode resistance in carrot germplasm. In this project we will confirm the strength of the resistance in cooperation with University of California nematologists, intercross best resistance, produce ample seed for extensive field testing, and release best resistance to the public, especially growers and seed companies. We will also evaluate horticultural quality including field performance traits (color, shape, uniformity) and consumer quality (flavor and nutritional value), hold field days for the carrot production community, and confirm genetic models of resistance in mapping populations.
Breeding populations were advanced and molecular markers evaluated to identify a new nematode resistance gene from Middle Eastern carrots. Field evaluation of resistance demonstrated consistent high levels of resistance in selected inbreds and hybrids. Nutritional quality evaluations have been made based upon carotenoid and anthocyanin pigment levels and types, and promising nutritional value is equivalent to or surpasses average carotene content of major current U.S. carrot cultivars, with typical anthocyanin and lutein content compared to several purple and yellow carrots grown in the U.S. Flavor evaluations indicated flavor comparable to standard U.S. carrots, with exceptional juicy texture noted in some selections. Germplasm is being released and large-scale testing is initiated.
This research relates to Objective 1, Determine the genetic basis of and initiate selection for carrot, onion, cucumber, and melon quality attributes influencing human nutrition and health, disease resistances, and yield and quality components, and stress tolerance in cucurbits, and perform field performance and quality trials and Objective 2, Utilize current biotechnology to discover and evaluate genetic variation and to map agriculturally important traits in Allium, Cucurbit, and Daucus germplasm, and to develop genetic and breeding stocks.