2013 Annual Report
To further characterize the molecular basis of CSFV and host-cell interactions ARS, PIADC and the University of Connecticut will identify swine macrophage proteins interacting with structural and non-structural CSFV proteins during infection. The effect(s) of these interactions on virulence, generalization of infection, tissue tropism, virus transmission, immunogenicity and induction of protection will be determined in swine.
2. The University of Connecticut will conduct fine mapping of interacting host and viral proteins to identify specific binding residues or motifs mediating the interaction.
3. Mutant viruses harboring genetically modified binding motifs will be constructed and characterized in vitro and in vivo at ARS, PIADC. Particular emphasis will be placed on establishing the ability of mutant viruses to cause disease and to induce protection in swine, relative to parental virulent virus.
Introduced specially designed mutations that partially substitute some of the 15 amino acid residues composing the E2 putative FP. Substitutions within E2 were introduced in the context of a full-length DNA copy of CSFV strain Brescia (pBIC) to obtain mutant viruses lacking portions of the FP of E2. Some of these substitutions affected in a differential manner the production of E2 protein in infected cells as well as the yield of viral progeny. No technologies were transferred or publications produced in FY 2013.