1a.Objectives (from AD-416):
Identify sources of seedling and adult plant resistance to wheat stem and leaf rust, and oat crown rust; Map resistance genes and QTLs for resistance to cereal rusts; and Use molecular and virulence markers to determine pathogen population structure and migration patterns.
1b.Approach (from AD-416):
Screen oat and wheat seedlings for rust resistance in the greenhouse; Screen oat and wheat adult plants for resistance in the field to rusts and FHB; Screen recombinant inbred (RI) populations for rust resistance in the greenhouse and field; and Use DArT and SSR markers to genotype RI populations; Determine virulence phenotype and molecular (SSR) genotype of rust and FHB pathogen isolates from the U.S.
Metagenomic approaches have been used to access healthy plant and soil microbial communities and to measure changes in microbial composition in response to biotic perturbations such as agricultural cultivation practices and fungicide treatment. We have conducted experiments designed to test the effects of plant species diversity on the composition of plant associated soil communities and the composition of fungi found within and upon healthy wheat. Preliminary results have indicated a marked difference in microbial community structure in healthy grasses compared with those associated with other plant species. Our results will be useful to plant pathologists and plant improvement specialists in efforts to develop strategies that maintain plant health.