2011 Annual Report 1a.Objectives (from AD-416) This research will identify steroid profiles for fish used in spawning efforts. The goal is to identify patterns and concentrations of steroids in these fish to provide better management tools to producers. 1b.Approach (from AD-416) Sera samples from fish will be collected and the steroid profiles compared. Reproductive success will be measured and correlated to steroid profiles. 3.Progress Report The goals for the reporting period include the analysis of fish samples for steroid content in plasma. Samples from Auburn and Mississippi are being examined for steroid composition using High Pressure Liquid Chromatography Mass Spectrometry (HPLC/MS) and HPLC-MS/MS. We are largely focusing on T3 and T4 steroids associated with male reproduction. We have completed about 35% of the total sample number provided from Auburn, no samples have been provided from Mississippi. We additionally have completed the analysis of bony chub samples from Arizona State University for steroid content as a potential grant proposal effort. The ADODR monitors this project through site visits, telecoms, and emails to the collaborator, and through annual reports from the collaborator.