1a.Objectives (from AD-416):
This research will identify steroid profiles for fish used in spawning efforts. The goal is to identify patterns and concentrations of steroids in these fish to provide better management tools to producers.
1b.Approach (from AD-416):
Sera samples from fish will be collected and the steroid profiles compared. Reproductive success will be measured and correlated to steroid profiles.
The goals for the reporting period include the analysis of fish samples for steroid content in plasma. Samples from Auburn, AL, and Mississippi were examined for steroid composition using High Pressure Liquid Chromatography Mass Spectrometry (HPLC/MS) and HPLC-MS/MS. We focused on T3 and T4 steroids associated with male reproduction. We have completed all samples sent from Auburn, no samples have been provided from Mississippi. Results from this project have been provided to Southern Regional Aquacultural Center. We additionally have completed the analysis of bony chub samples from Arizona State University for steroid content as a potential grant proposal effort. A new to science algal toxin was published –this toxin was also patented by ARS. A new method for analysis of water soluble pigments in cyanobacteria was developed and published.