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United States Department of Agriculture

Agricultural Research Service

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Research Project: ULTRA-FAST METHODS AND INSTRUMENTATION FOR THE ANALYSIS OF HAZARDOUS CHEMICALS IN THE FOOD SUPPLY

Location: Residue Chemistry and Predictive Microbiology

2012 Annual Report


1a.Objectives (from AD-416):
The main objective is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods.

The proposed research is designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods with full analysis cycle time of < 1 min. We shall explore: a) unique high flow rate universal SMB-MS for gas chromatography; b) novel open probe SMB-MS/MS as an even faster alternative; c) liquid flow injection SMB-MS/MS to eliminate ion suppression effects; and d) fast SMB-MS for liquid chromatography. Appropriate sampling protocols and sample preparation methods will be devised for the ultra-fast detection approaches to ensure meaningful results. Emphasis will be given to address the needs of fast pesticide and veterinary drug analysis prior to food distribution and to meet the ultra-fast analytical demands of possible food terrorism scenarios.


1b.Approach (from AD-416):
We shall explore new and novel sample preparation approaches for the multiclass, multiresidue analysis of drug residues in foods. Prof. Amirav will further develop supersonic molecular beam mass spectrometry, particularly using liquid chromatography for the polar drug analytes rather than gas chromatography as was the case for pesticides.


3.Progress Report:

The main objective of this project is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods. The research is predominantly designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods. Prof. Aviv Amirav, the collaborator in Israel, has made excellent progress on the ultra-fast SMB-MS analysis component of the project with the goal of less than 1 min analysis time. During the third year in the ARS lab, we extended the “Goldilocks” method to twice as many veterinary drug residues in food animal tissues as in the previous year. More than 120 veterinary drugs have now been included in the method, which was developed, validated, and transferred to the USDA Food Safety Inspection Service. Further studies designed to optimize the extraction, cleanup, and detection of the veterinary drug residues in animal tissues were conducted, including a rigorous assessment of matrix effects on qualitative and quantitative analysis. A single analyst can process 60 samples per 8 hour day using this method followed by sequential analyses of 10 min each using ultra-performance liquid chromatography – triple quadrupole tandem mass spectrometry. This analytical method is capable of simultaneous identification and quantification of >100 veterinary drugs at regulatory tolerance levels in beef kidney, muscle, and other tissue types. The project is on schedule for completion at the end of the fiscal year.


Last Modified: 9/2/2014
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