Development of Molecular Markers to Identify Different Physiological Races of Pathogens Responsible for Soilborne Diseases of Alfalfa
Plant Germplasm Introduction and Testing
2013 Annual Report
1a.Objectives (from AD-416):
Identify molecular markers specific to Aphanomyces eiteiches Race 1 and 2.
1b.Approach (from AD-416):
Collect fungal isolates from various geographical regions. Extract DNA from each and combine from each respective race (bulked DNA). Use RAPD, SRAP, or other primers to screen for polymorphisms specific to each race. Develop SCAR markers from sequence derived from the latter markers. Documents SCA with WSU.
Due to sequester and recision cuts, this SCA was unfunded this FY. Project is on hold. This project contributes to in-house Objective 1: "Develop molecular markers to screen alfalfa for resistance to environmental stresses and identify different physiological races of pathogens responsible for soilborne diseases of alfalfa".