Enhancing the Efficiency of Induced Mutagenesis Through an Integrated Biotechnology
Crops Pathology and Genetics Research
2013 Annual Report
1a.Objectives (from AD-416):
The SY will serve in a technical advisor role to this IAEA-sponsored Coordinated Research Project (CRP) to share expertise in the area of rice genomics and induced mutagenesis with research contract holders from developing countries.
1b.Approach (from AD-416):
Involves participation in Research Coordination Meetings at the IAEA, Vienna Austria by presenting and discussing ongoing research in the SY's lab involving the development and analysis of rice mutants. Documents NFCA with IAEA.
The agreement was originally established in support of objectives 1 and 2 of the former in-house project 5306-21000-017-00D, GENETIC DISSECTION OF SEEDLING COLD TOLERANCE IN RICE (ORYZA SATIVA L.). This project supports objectives 1, 2, and 3 of the current in-house project 5306-21000-021-00D, GENERATION & CHARACTERIZATION OF NOVEL GENETIC VARIATION IN RICE FOR THE ENHANCEMENT OF GRAIN QUALITY AND AGRONOMIC PERFORMANCE, the overall goal being to employ classical mutagenesis to induce genetic variation in rice that will be exploited for functional genomics and variety development. The goal of the project involves participation in Research Coordination Meetings at the International Atomic Energy Agency in Vienna, Austria, by presenting and discussing ongoing research in the Scientist’s laboratory involving the development and analysis of rice mutants.
During the reporting period, research on the application of exome sequencing to evaluate rice mutants was conducted with cooperators at the University of California, Davis, and a manuscript describing this work is in preparation and will be submitted before the end of FY2013. Exome sequencing involves the targeting of those regions of the genome that encode proteins (i.e. exons; expressed sequences) for large-scale sequencing. Results indicate that this approach is effective for evaluating the mutation densities of populations and for characterizing mutants for functional genomics. Work on development of populations of rice mutants in the variety Kitaake continued with the harvesting of M4 seeds from approximately 200 M3 lines and the planting of the remaining M3 lines (2,000-3,000) for M4 seed production with harvest expected in December 2013.