Enhancing the Efficiency of Induced Mutagenesis Through an Integrated Biotechnology
Crops Pathology and Genetics Research
2011 Annual Report
1a.Objectives (from AD-416)
The SY will serve in a technical advisor role to this IAEA-sponsored Coordinated Research Project (CRP) to share expertise in the area of rice genomics and induced mutagenesis with research contract holders from developing countries.
1b.Approach (from AD-416)
Involves participation in Research Coordination Meetings at the IAEA, Vienna Austria by presenting and discussing ongoing research in the SY's lab involving the development and analysis of rice mutants.
The agreement was established in support of objectives 1 and 2 of the in-house project, the goal being to develop improved rice (Oryza sativa L.) germplasm for use in breeding elite varieties adapted to temperate environments by identifying, characterizing, and manipulating genes that affect crop productivity. The goal of this project involves participation in Research Coordination Meetings at the International Atomic Energy Agency in Vienna, Austria by presenting and discussing ongoing research in the Scientist’s laboratory involving the development and analysis of rice mutants. During the past year, M2 generation seeds from materials generated in FY10 were planted but no obvious mutant phenotypes were observed. Additional rice mutants were then generated by a visiting IAEA fellow using sodium azide mutagenesis of the very early-maturing rice varieties Kitaake. M2 seeds from the primary Kitaake mutants (M1) were obtained and visual phenotyping and DNA analysis using next-generation sequencing was performed in FY11 in cooperation with Luca Comai (UC Davis). By sequencing a representative fraction of the genomes of Kitaake M2 mutants generated using different sodium azide treatments we were able to assess the mutation density of these mutants. This work shows that this approach is feasible for rapidly and economically assessing the effectiveness of mutagens in generating mutations and will be useful for evaluating the potential of a mutant population for use as a reverse genetics resource prior to investigating significant time and resources. In addition, this approach can be used with or without reference genome sequence, thus making it amenable to those working with species which have received little attention or funding. A manuscript is in preparation and will be submitted in FY11. This work is being extended to examine the genetic effective cell number by analyzing M2 plants derived from the same M1 panicle which is expected to be completed in the first quarter of FY12.
The project is monitored by maintaining a complete file of the agreement, reviewing the annual reports, and conducting meetings with the cooperator during the course of the agreement.